Read e-book online Current research in embryology PDF

By Sabine Globig

ISBN-10: 1926692810

ISBN-13: 9781926692814

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Hsp105 protein was observed mainly in the luminal epithelium on day 1 of pregnancy (D1) and moderately expressed in the luminal epithelium and the glandular epithelium from day 2 to day 3 (D2). Hsp105 staining was also detected in the stromal cells, immediately underneath the luminal epithelium on day 4 and day 5 of pregnancy (D4, D5), the staining was increased markedly on day 5 just before implantation (D5). On day 6, the protein staining was mainly observed in the implanted blastocyst and the stromal cells around the implantation site (D6), while its expression in the luminal epithelium reduced to an undetected level.

CON, negtive control; le, luminal epithelium; ge, glandular epithelium; s, stroma; de, decidua; em, embryo. Bar = 200 μm. 54 Current Research in Embryology Table 1. Semi-quantitative estimation of Hsp105 expression in the various uterine cells during early pregnancy Western Blot Analysis of Hsp105 Expression in Uterus During Early Pregnancy The quantitative change in uterine Hsp105 expression was estimated by Western blot, as shown in Fig. 2. The protein level in the uterus was increased in a timedependent manner, the highest expression was observed on day5 and day 6, just around the time before and after implantation.

40. Reece EA, Pinter E, Homko C, Wu Y-K, Naftolin F: The Yolk Sac Theory: Closing the Circle on Why Diabetes-Associated Malformations Occur. J Soc Gynecol Investig 1994, 1(1):3–13. 41. Reece EA, Ji I, Wu YK, Zhao Z: Characterization of differential gene expression profiles in diabetic embryopathy using DNA microarray analysis. Am J Obstet Gynecol 2006, 195(4):1075–1080. 42. Goto MP, Goldman AS: Diabetic embryopathy. Curr Opin Pediatr 1994, 6(4):486–491. 43. Fine EL, Horal M, Chang TI, Fortin G, Loeken MR: Evidence that elevated glucose causes altered gene expression, apoptosis, and neural tube defects in a mouse model of diabetic pregnancy.

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Current research in embryology by Sabine Globig


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