M. E. Drets, G. A. Folle, F. J. Monteverde (auth.),'s Chromosomal Aberrations: Basic and Applied Aspects PDF

By M. E. Drets, G. A. Folle, F. J. Monteverde (auth.), Professor Dr. Günter Obe, Professor Dr. A. T. Natarajan (eds.)

ISBN-10: 3642756824

ISBN-13: 9783642756825

ISBN-10: 3642756840

ISBN-13: 9783642756849

Chromosomale Mutationen sind eine der m|glichen Ursachen f}r Ver{nderungen der Erbinformation. Neben grunds{tzlichen As- pekten, wie Reparaturmechanismen der Zelle oder Ursachen von Chromosomenver{nderungen, werden angewandte Aspekte, z.B. Chromosomen als Testindikatoren der Toxizit{t, behandelt.

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Extra resources for Chromosomal Aberrations: Basic and Applied Aspects

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Schematic illustration of the experimental approach. Purified poly(ADP-ribose)polymerase was incubated for various intervals with a 146-bp core DNA fragment in the presence of [32 PJNAD +. The radiolabeled polymers were chemically detached from the enzyme molecules, purified by boron ate affinity chromatography, and separated into individual polymer size classes. The amount of radioactivity in each polymer class was quantified. The relative size distribution patterns at different stages of the reaction were analyzed using three-dimensional graphics microcomputer software 60 40 :3IJ -zO ~o 60 ~o Fig.

NAEGELI, P. PANZETER, and C. REALINll Contents Introduction .... . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 2 Enzymatic Components of the Poly-ADP-Ribosylation System ........................ 3 Mode of Polymer Addition to Proteins ............................................. 4 In Vitro System to Study Shuttling by Poly-ADP-Ribosylation .. , . . . . . . . . . . .. 5 Conclusions . . . . . . . . . . . . . . . . . . . .

Was about 30%, which was comparable to that for log-phase cells without any pretreatment. The plating efficiency of cells treated with colcemid was 20-50 % of untreated cells. The experiment was carried out four times, using the same doses as for the measurement of chromosome aberrations, with five replicate dishes/data point. 0 44 R. C. Moore et al. 3 Results The yield of chromatid exchanges as a function of dose is shown in Fig. 1. The data was fitted to the expression y = aD-bD 2, using the method of conjugate search (Powell 1964), and assuming the errors were Poisson-distributed.

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Chromosomal Aberrations: Basic and Applied Aspects by M. E. Drets, G. A. Folle, F. J. Monteverde (auth.), Professor Dr. Günter Obe, Professor Dr. A. T. Natarajan (eds.)


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